JCM 5046 PDFJCM 5046 PDF

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Frequency of BKCproducing Klebsiella species jccm. Please review our privacy policy. Initially, we performed transformation by electroporation into Escherichia coli Top In this study, a low frequency of isolates carrying bla BKC-1 was observed and was mainly attributed to the persistence of a K.

This article has been cited by other articles in PMC. The two BKCproducing isolates belonged to clonal complex and possessed identical pulsed-field gel electrophoresis 546.

Published online Jul The other authors have no conflicts to declare.

Prepublished online May Only 2 of Klebsiella isolates 0. Antimicrob Agents Chemother Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard— 10th ed CLSI document MA The Klebsiella strains were isolated from different clinical jck obtained between and Fig. To date, inactivation of mgrB has been the main mechanism of polymyxin resistance observed in K.

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In the past decade, carbapenemase-encoding genes such as bla KPC-likebla OXAand bla NDM-like have emerged as the main mechanisms of carbapenem resistance in Enterobacteriaceae.

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S, sensitive; R, resistant. Transformant cells were selected on LB agar plates supplemented with 0. Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines 45.

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The Kpn and KpnJ strains showed different plasmid profiles. Kpn showed susceptibility to tigecycline MIC, 0. Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for jjcm nosocomial dominance.

BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates.

Journal List Antimicrob Agents Chemother v. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only effective tools for preventing the acquisition and spread of new carbapenemase-encoding genes.

More studies with greater numbers of isolates from all Brazilian states are necessary to assess the real prevalence of isolates carrying bla BKC The jcmm BKC-1 gene was inserted into a kb plasmid that was identical to the previously reported plasmid, p Sampaiob, c and Ana C. Plasmid extraction of K. Performance standards for antimicrobial susceptibility testing; 25th informational supplement.

Author information Article notes Copyright and License information Disclaimer. Santosb Jorge L. The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniae. The principal objective of this study was to evaluate the frequency of bla BKC-1 by testing a collection of Klebsiella isolates. Diagn Microbiol Infect Dis J Antimicrob Chemother Martinsa Adriana G. Open in a separate window. To evaluate the stability of p harbored by the Kpn isolate, we performed serial passages of the Kpn isolate on Mueller-Hinton agar plates without the addition of antibiotic.

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Among the Klebsiella strains, Only 2 BKCproducing K. Clinical and Laboratory Standards Institute.

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Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.

Support Center Support Center. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates.

Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. Antimicrobial susceptibility profiles of BKCproducing K.

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However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. For comparison purposes, the first reported BKC-producing strains, i.

J Clin Microbiol